Limitations of modified substrate recognition by Taq DNA polymerase mutants (967.2)
نویسندگان
چکیده
منابع مشابه
Taq DNA Polymerase Mutants and 2'-Modified Sugar Recognition.
Chemical modifications to DNA, such as 2' modifications, are expected to increase the biotechnological utility of DNA; however, these modified forms of DNA are limited by their inability to be effectively synthesized by DNA polymerase enzymes. Previous efforts have identified mutant Thermus aquaticus DNA polymerase I (Taq) enzymes capable of recognizing 2'-modified DNA nucleotides. While these ...
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1.Belgrader, P., W. Benett, D. Hadley, G. Long, R. Mariella, Jr., F. Milanovich, S. Nasarabadi, W. Nelson et al. 1998. Rapid pathogen detection using a microchip PCR array instrument. Clin. Chem. 44:2191-2194. 2.Belgrader, P., J.K. Smith, V.W. Weedn and M.A. Northrup. 1998. Rapid PCR identity testing using a battery-powered miniature thermal cycler. J. Forensic Sci. 43:315-319. 3.Belinda, A.J.G...
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The Applied Biosystems PRISM fluorescence-based genotyping system as well as the Invitrogen TA Cloning vector system are influenced by the tendency of Taq DNA polymerase to add an adenine nucleotide to the 3' end of PCR products after extension. Incomplete addition of adenine to a majority of PCR product strands creates problems in allele-calling during genotyping and potentially diminishes the...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2014
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.28.1_supplement.967.2